Structure of Chymotrypsin EC 3.4.21.1 CAS 9004 07 3 - Chymotrypsin EC 3.4.21.1 CAS 9004-07-3

Iden­ti­fi­ca­tion

CAS Number

9004-07-3

EC Number

3.4.21.1

Name

Chy­motrypsin

Syn­onyms

quimo­trase ;
zol­yse ;
TLCK-CHY­­MOTRYPSIN ;
ALPHA-CHY­­MOTRYPSIN, TLCK TREAT­ED ;
ALPHA-CHY­­MOTRYPSIN TYPE I-S ;
ALPHA-CHY­­MOTRYPSIN TYPE VI ;
ALPHA-CHY­­MOTRYPSIN TYPE VII ;
ALPHA-CHY­­MOTRYPSIN

EINECS

232-671-2

MDL Number

MFCD00130481

Prop­er­ties

Appear­ance

White or off white powder

Safe­ty Data

Sym­bol

exclamation mark jpg - Chymotrypsin EC 3.4.21.1 CAS 9004-07-3health hazard jpg - Chymotrypsin EC 3.4.21.1 CAS 9004-07-3GHS07,GHS08

Sig­nal Word

Dan­ger

Haz­ard statements

H315H319H334H335

Pre­cau­tion­ary Statements

P302 + P352P305 + P351P338

Tar­get organs

Res­pi­ra­to­ry system

Per­son­al Pro­tec­tive Equipment

dust mask type N95 (US), Eye­shields, Faceshields, Gloves

RIDADR 

NONH for all modes of transport

WGK Germany

1

RTECS

GC3050000

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Chy­motrypsin EC 3.4.21.1 CAS 9004-07-3

NameSequenc­ing grade chymotrypsinRecom­bi­nant Human Chymotrypsin
Spe­cif­ic activity≥1500 USP units/​mg pro.≥1000 USP units/​mg pro.
SDS-PAGESin­gle major bandSin­gle major band
SDS-PAGE (MW)24.0±2.4 kDa24.0±2.4 kDa
Puri­ty(HPLC)≥ 95%
Unit Def­i­n­i­tionOne unit will hydrolyze 1.0 μmol of N-alpha-acetyl-L-tyro­­sine eth­yl ester(ATEE) per min at pH 7.0 at 25 °C.
Rec­om­mend­ed usagePre­pare 1-10mg/ml sequenc­ing grade chy­motrypsin with 50mM HAC.When digest the pro­tein, the tar­get pro­tein was dis­solved with 50 mM or 100 mM NH4HCO3, and then add a cer­tain amount of enzyme.The ratio to aimed pro­tein is 1:50 to 1:1000 (w/​w) at 30℃.When the tem­per­a­ture is more than 30℃,enzyme will be self-cutting.Pre­pare 1-10mg/ml recom­bi­nant human chy­motrypsin with 1mM HCl.The ratio to aimed pro­tein is 1:50 to 1:1000 (w/w).The opti­mum pH is pH7.0-9.0.
Sta­bil­i­ty of storageSequenc­ing grade chy­motrypsin lyophilized should be stored under 2-8℃ in sealed con­tain­er. It is sta­ble with­in 24 months.After dis­solv­ing with 50mM HAc, store at 2-8 ℃,or use with­in 2 days.If the solu­tion is stored at -20 ℃ or below, it is sta­ble with­in 24 months. The solu­tion should avoid repeat­ed freez­ing and thaw­ing and plac­ing at room tem­per­a­ture a long time.Recom­bi­nant human chy­motrypsin lyophilized should be stored under 2-8℃ in sealed con­tain­er. It is sta­ble with­in 24 months.After dis­solved with 1mM HCl , it should be stored under -20℃. Avoid repeat­ed freez­ing and thawing.
Sta­bil­i­ty of transportThe prod­uct is sta­ble by blue ice insu­la­tion transport.The prod­uct is sta­ble by blue ice insu­la­tion transport.
Appli­ca­tionUsed alone or in com­bi­na­tion with oth­er pro­teas­es to pro­duce pro­tein digests for pep­tide map­ping appli­ca­tions or pro­tein iden­ti­fi­ca­tion by pep­tide mass fin­ger­print­ing or MS/MS spec­tral match­ing. It is suit­able for diges­tion reac­tions in-solu­­tion or in-gel.Used alone, or with oth­er pro­teas­es to inter­act with diges­tive pro­teins, or oth­er spe­cial uses such as organ­ic synthesis.

Descrip­tion

It is a recom­bi­nant ser­ine endopep­ti­dase expressed in E.coli, puri­fied with HPLC, the gene sequence is the same as human chy­motrypsin. Sequenc­ing grade chy­motrypsin hydrolyzes at the car­boxyl side of aro­mat­ic amino acids residues : Tyr, Phe and Trp. Cleav­age may also be observed, but at a low­er rate, at Leu and Met. The opti­mal pH is 7.0 – 9.0.

Source

Recom­bi­nant E. coli

Pro­tein Sequence

The amino acid sequence of sequenc­ing grade chy­motrypsin is iden­ti­cal to human chymotrypsin

Advan­tage

  1. Ani­mal ori­gin free : recom­bi­nant trypsin is no exoge­nous virus contamination,and any ani­mal ori­gin mate­r­i­al is not used in the pro­duc­tion process.
  2. Sta­ble qual­i­ty : Mass pro­duc­tion can ensure sta­ble and con­tin­u­ous batch pro​duc​tion​.It is no dif­fer­ence between the batch and the prod­uct qual­i­ty is stable.
  3. High puri­ty : High­er spe­cif­ic activ​i​ty​.Host pro­tein residues is less than the lim­its of bio­log­i­cal products.
  4. Lyophilized pow­der : The prod­uct is lyophilized pow­der and is easy to store and transport.
This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd(https://​www​.wat​son​-bio​.com/), and here is the cor­re­spond­ing linkhttps://​www​.wat​son​-bio​.com/​c​h​y​m​o​t​r​y​p​s​i​n​-​e​c​-​3​-​4​-​2​1​-​1​-​c​a​s​-​9​0​0​4​-​0​7​-3/

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