20230426095246 600x400 - Human PRAME (HLA-A*02:01) Tetramer Protein, Accession: A0A140T913

Overview

Gen­er­al Description

Recom­bi­nant Human PRAME (HLA-A*02:01) Tetramer Pro­tein is expressed from HEK293 with His tag and Avi tag at the C-ter­mi­­nal, tetramer is assem­bled by biotiny­lat­ed monomer and strep​ta​vidin​.It con­tains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLQH­LIGL peptide.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

PRAME (HLA-A*02:01)

Syn­onyms

Acces­sion

A0A140T913

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M)and SLLQH­LIGL peptide

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 258 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 260-265 kDa under Non reduc­ing (N) con­di­tion based on Tris-Bis PAGE result.

Activ­i­ty

Prod­uct Tag

C-His-Avi

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Liq­uid

Ship­ping Conditions

Shipped with blue ice.

For­mu­la­tion

Sup­plied as 0.22um fil­tered solu­tion in PBS (pH 7.4).

Recon­sti­tu­tion

Sta­bil­i­ty & Storage

Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the pro­tein into small­er quan­ti­ties for opti­mal stor­age. Please avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

20230426095246 - Human PRAME (HLA-A*02:01) Tetramer Protein, Accession: A0A140T913

The puri­ty of Human PRAME (HLA-A*02:01) Tetramer is greater than 95% as deter­mined by SEC-HPLC.

20221125165412 - Human PRAME (HLA-A*02:01) Tetramer Protein, Accession: A0A140T913

Human PRAME (HLA-A*02:01) Tetramer on Tris-Bis PAGE under Non reduc­ing (N) con­di­tion. The puri­ty is greater than 95%.

validation images - Human PRAME (HLA-A*02:01) Tetramer Protein, Accession: A0A140T913

validation images - Human PRAME (HLA-A*02:01) Tetramer Protein, Accession: A0A140T913

validation images - Human PRAME (HLA-A*02:01) Tetramer Protein, Accession: A0A140T913

validation images - Human PRAME (HLA-A*02:01) Tetramer Protein, Accession: A0A140T913

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