Overview

General Description

Recombinant Biotinylated Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Glu22-Cys213.

Biotinylated

Yes

Specifications

Target

IL-2 R alpha/CD25

Synonyms

CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR

Accession

P01589

Species

Human

Exact Sequence

Glu22-Cys213

Molecular Weight

The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-CD25 Antibody, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

General View of Documents

Immobilized Anti-CD25 Antibody, hFc Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA.

The purity of Biotinylated Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.

Biotinylated Human IL-2 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).

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