Overview

Gen­er­al Description

Recom­bi­nant Biotiny­lat­ed Human P53 R175H (HLA-A*02:01) Pro­tein is expressed from HEK293 with His tag and Avi tag at the C​-ter​mi​nal​.It con­tains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and HMTEVVRHC peptide.

Biotiny­lat­ed

Yes

Spec­i­fi­ca­tions

Tar­get

P53 R175H (HLA-A*02:01)

Syn­onyms

MHC ; HLA-A ; P53 ; TP53 ; Anti­gen NY-CO-13 ; BCC7 ; FLJ92943 ; LFS1 ; TRP53

Acces­sion

A0A140T913

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and HMTEVVRHC peptide

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 50.5 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 55-60 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Anti-P53 R175H (HLA-A*02:01) Anti­body, hFc Tag at 5ug/​ml (100ul/​well) on the plate. Dose response curve for Biotiny­lat­ed Human P53 R175H (HLA-A*02:01) , His Tag with the EC50 of 1.6ug/ml deter­mined by ELISA. See test­ing image for detail.

Prod­uct Tag

C-His-Avi

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS, 100mM L-argi­­nine (pH 7.4). Nor­mal­ly 8% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Immo­bi­lized Anti-P53 R175H (HLA-A*02:01) Anti­body, hFc Tag at 5ug/​ml (100ul/​well) on the plate. Dose response curve for Biotiny­lat­ed Human P53 R175H (HLA-A*02:01) , His Tag with the EC50 of 1.6ug/ml deter­mined by ELISA.

The puri­ty of Biotiny­lat­ed Human P53 R175H (HLA-A*02:01) was greater than 95% as deter­mined by SEC-HPLC.

Biotiny­lat­ed Human P53 R175H (HLA-A*02:01) on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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