20211025155030 600x400 - Biotinylated Human SIRP alpha V3 Protein, Accession: ATD50864

Overview

Gen­er­al Description

Recom­bi­nant Biotiny­lat­ed Human SIRP alpha V3 Pro­tein is expressed from HEK293 with His tag and Avi tag at the C​-ter​mi​nal​.It con­tains Glu31-Arg369(H54L).

Biotiny­lat­ed

Yes

Spec­i­fi­ca­tions

Tar­get

SIRP alpha V3

Syn­onyms

CD172a ; BIT ; MFR ; MYD1 ; MYD-1 ; P84 ; PTPNS1 ; SHP sub­strate 1 ; SHPS1 ; SHPS-1 ; SHPS1CD172A ; SIRP alpha ; SIR­PA ; Sirp-alpha-1 ; SIRPalpha2 ; Sirp-alpha-2 ; Sirp-alpha-3

Acces­sion

ATD50864

Species

Human

Exact Sequence

Glu31-Arg369(H54L)

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 39.8 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 55-65 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Biotiny­lat­ed Human SIRP alpha V3, His Tag at 1ug/​ml (100ul/​Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.12ug/ml deter­mined by ELISA. See test­ing image for detail.

Prod­uct Tag

C-His-Avi

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 8% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

20211025155030 - Biotinylated Human SIRP alpha V3 Protein, Accession: ATD50864

Immo­bi­lized Biotiny­lat­ed Human SIRP alpha V3, His Tag at 1ug/​ml (100ul/​Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.12ug/ml deter­mined by ELISA.

20210927151838 - Biotinylated Human SIRP alpha V3 Protein, Accession: ATD50864

The puri­ty of Biotiny­lat­ed Human SIRP alpha V3 is greater than 95% as deter­mined by SEC-HPLC.

20210927151832 - Biotinylated Human SIRP alpha V3 Protein, Accession: ATD50864

Biotiny­lat­ed Human SIRP alpha V3 on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

validation images - Biotinylated Human SIRP alpha V3 Protein, Accession: ATD50864

validation images - Biotinylated Human SIRP alpha V3 Protein, Accession: ATD50864

validation images - Biotinylated Human SIRP alpha V3 Protein, Accession: ATD50864

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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