Overview

General Description

Recombinant Human HLA-E*01:03 Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M) and VMAPRTLVL peptide.

Biotinylated

Specifications

Target

HLA-E*01:03 Tetramer

Synonyms

HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1

Accession

P13747

Species

Human

Exact Sequence

Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M)and VMAPRTLVL peptide

Molecular Weight

The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Activity

The affinity constant of 17.61 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

General View of Documents

Human NKG2A&CD94, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 17.61 nM as determined in SPR assay (Biacore T200) (QC Test).

Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Tetramer, His Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200).

The purity of Human HLA-E*01:03 Tetramer is greater than 95% as determined by SEC-HPLC.

Human HLA-E*01:03 Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).

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