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Recombinant Human TNFRSF12A/TWEAKR Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Glu28-Per80.
FGF-inducible 14 ; Fgfrp2 ; TNFRSF12A ; FN14 ; CD266 ; TWEAKR
The protein has a predicted MW of 32.9 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
Immobilized Human TNFRSF12A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.24 nM as determined in SPR assay (Biacore T200). See testing image for detail.
> 95% as determined by Tris-Bis PAGE
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Shipped at ambient temperature.
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Human TNFSF12, hFc Tag immobilized on CM5 Chip can bind Human TNFRSF12A, hFc Tag with an affinity constant of 0.24 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNFRSF12A, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-TNFRSF12A Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test).
Human TNFRSF12A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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