Offi­cial Full Name

Recom­bi­nant Fla­gellin, His (rFlagellin,His)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

661-02-1816

Syn­onyms

Squence

Squence of Recombinant Flagellin, His

Amino Acid Sequence

MAQV­INTNSL SLLTQNNLNK SQSAL­GTAIE RLSS­GLRINS AKD­DAAGQAI ANRF­TANIKG LTQAS­R­NAND GISI­AQT­TEG ALNEINNNLQ RVRELAVQSA NSTNSQS­DLD SIQAEITQRL NEI­DRVS­GQT QFNGVKVLAQ DNTL­TIQV­GA NDGE­TI­DIDL KQIN­SQTL­GL DTL­NVQQKYK VSD­TAATVTG YADT­TIALDN STFKASAT­GL GGT­DQKIDGD LKFD­DTTGKY YAKVTVTG­GT GKDGYYEVSV DKT­NGEVT­LA GGAT­SPLTGG LPATAT­ED­VK NVQ­VANADLT EAKAAL­TAAG VTG­TASVVKM SYTDNNGK­TI DGGLAVKVGD DYYSATQNKD GSIS­INT­TKY TAD­DGT­SK­TA LNKLGGADGK TEVVSIG­GKT YAASKAEGHN FKAQPD­LAEA AATT­TEN­PLQ KIDAALAQVD TLRS­DL­GAVQ NRFN­SAITNL GNTVNNLT­SA RSRIEDS­DYA TEVS­N­M­SRAQ ILQQAGTSVL AQAN­QVPQNV LSLL­R­LEHHH HHH

Syn­onyms

Acces­sion

P06179

Gene ID

1253480

Sum­ma­ry

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 52.7 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 503 amino acids, with Leu, Glu and 6 × His at C-ter­mi­nus.

Bio­log­i­cal Activity

No data avail­able

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in PBS, pH 7.4.

Endo­tox­in

Less than 1 EU/​ug of rFla­gellin, His as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

1. Fedorov OVand­Kostyuko­va AS. 1984. FEBS Lett, 171 : 145-8.2. Belas Rand­Fla­her­ty D. 1994. Gene, 148 : 33-41.3. Givau­dan A, Lanois A, Boe­mare N. 1996. Gene, 183 : 243-53.4. Meindl T, Boller T, Felix G. 2000. Plant Cell, 12 : 1783-94.5. Hayashi F, Smith KD, Ozin­sky A, et al. 2001. Nature, 410 : 1099-103.

Puri­ty

> 95 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

SDS-PAGE of Recombinant Flagellin, His

Pack Size

10ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Fla­gellin, His (rFlagellin,His) on ChemWhat

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