Recom­bi­nant Human Fibrob­last Growth Fac­tor 19 (rHuFGF-19) CAS 104-19-1816

Offi­cial Full Name

Recom­bi­nant Human Fibrob­last Growth Fac­tor 19 (rHuFGF-19)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

104-19-1816

Syn­onyms

Squence

Amino Acid Sequence

MRPLAFS­DAG PHVHYG­WGDP IRL­RHLYTSG PHGLSS­CFLR IRADGVVD­CA RGQSAH­SLLE IKAVAL­RT­VA IKGVHSVRYL CMGADGK­MQG LLQY­SEED­CA FEEEIR­PDGY NVYRSEKHRL PVSLSSAKQR QLYKN­RGFLP LSH­FLPMLPM VPEEPEDL­RG HLES­DMF­SSP LETDSMDPFG LVT­GLEAVRS PSFEK

Syn­onyms

Acces­sion

O95750

Gene ID

9965

Sum­ma­ry

Source

Mol­e­c­u­lar Weight

Approx­i­mate­ly 21.8 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 195 amino acids.

Bio­log­i­cal Activity

Assay #1 : Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The bio­log­i­cal activ­i­ty is mea­sured by its bind­ing abil­i­ty in a func­tion­al ELISA. Immo­bi­lized rHuFGF R4 at 5 ug/​ml can bind rHuFGF-19 with a lin­ear range of 3-200 ng/ml.Assay #2 : Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by a cell pro­lif­er­a­tion assay using murine Balb/​c 3T3 cells is less than 150 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 6.7 × 103 IU/​mg.

Appear­ance

Ster­ile Fil­tered White lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in PBS, pH 7.4.

Endo­tox­in

Less than 1 EU/​ug of rHuFGF-19 as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

1. Lad­her RK, Anakwe KU, Gur­ney AL, et al. 2000. Sci­ence. 290:1965-7.2. Tom­lin­son E, Fu L, John L, et al. 2002. Endocrinol­o­gy. 143:1741-7.3. Small­wood PM, Munoz-San­juan I, Tong P, et al. 1996. Proc Natl Acad Sci U S A. 93:9850-7.4. Fu L, John LM, Adams SH, et al. 2004. Endocrinol­o­gy. 145:2594-603.5. Khari­to­nenkov A, Shiyano­va TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35.6. Kuro­su H, Kuro OM. 2009. Mol Cell Endocrinol. 299:72-8.7. Lin BC, Wang M, Black­more C, et al. 2007. J Biol Chem. 282:27277-84.8. Khari­to­nenkov A, Dun­bar JD, Bina HA, et al. 2008. J Cell Phys­i­ol. 215:1-7.

Puri­ty

> 95 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

5ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

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Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Human Fibrob­last Growth Fac­tor 19 (rHuFGF-19) on ChemWhat

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