Recom­bi­nant Human Fms-relat­ed Tyro­sine Kinase 3 Lig­and (rHu­Flt-3Li­gand) CAS 103-05-1816

Offi­cial Full Name

Recom­bi­nant Human Fms-relat­ed Tyro­sine Kinase 3 Lig­and (rHu­Flt-3Li­­gand)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

103-05-1816

Syn­onyms

Flt3L, SL Cytokine

Squence

Amino Acid Sequence

TQD­CS­FQH­SP ISS­D­FAVKIR ELS­DYL­LQDY PVT­VASNLQD EEL­CG­GLWRL VLAQR­WMERL KTVAGSKMQG LLERVN­TEIH FVTK­CAFQPP PSCLR­FVQTN ISR­L­LQETSE QLVALKP­WIT RQN­F­S­R­CLEL QCQPDSSTLP PPWSPRPLEA TAP­TA

Syn­onyms

Flt3L, SL Cytokine

Acces­sion

P49771

Gene ID

2323

Sum­ma­ry

Flt3-lig­and (FL) is a recent­ly iden­ti­fied hematopoi­et­ic cytokine whose activ­i­ties are medi­at­ed by bind­ing to the trans­mem­brane gly­co­pro­tein Flt3. Flt3 was first dis­cov­ered as a mem­ber of the class III sub­fam­i­ly of recep­tor tyro­sine kinas­es (RTK) whose expres­sion among hematopoi­et­ic cells was found to be restrict­ed to high­ly enriched stem/​progenitor cell pop­u­la­tions. Addi­tion­al­ly, class III RTKs include the recep­tors from SCF, M-CSF and PDGF. Not sur­pris­ing­ly, Flt3-lig­and is also struc­tural­ly relat­ed to M-CSF and SCF. All three cytokines have been shown to exist both as type I trans­mem­brane pro­teins and as sol­u­ble pro­teins. The pre­dom­i­nant human FL iso­form is a trans­mem­brane pro­tein that can under­go pro­te­olyt­ic cleav­age to gen­er­ate a sol­u­ble form of the pro­tein. An alter­­na­­tive­­ly-spliced FL mRNA, encod­ing a sol­u­ble form of the human FL, has also been iden­ti­fied. FL is wide­ly expressed in var­i­ous human and mouse tis­sues. At the amino acid sequence lev­el, human and mouse FL are approx­i­mate­ly 72 % iden­ti­cal and the two pro­teins exhib­it cross-species activ­i­ty. FL has been shown to syn­er­gize with a wide vari­ety of hematopoi­et­ic cytokines to stim­u­late the growth and dif­fer­en­ti­a­tion of ear­ly hematopoi­et­ic prog­en­i­tors.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 17.6 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 155 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by a cell pro­lif­er­a­tion assay using human AML5 cells is less than 1.0 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 1.0 × 106 IU/​mg.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in PBS, pH 7.0.

Endo­tox­in

Less than 1 EU/​ug of rHu­Flt3-Lig­and as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

Puri­ty

> 97 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

10ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Human Fms-relat­ed Tyro­sine Kinase 3 Lig­and (rHu­Flt-3Li­­gand) on ChemWhat

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