Recom­bi­nant Human Indi­an Hedge­hog Cys28IleIle (rHuIHHC28II) CAS 601-411-1816

Offi­cial Full Name

Recom­bi­nant Human Indi­an Hedge­hog Cys28IleIle (rHuIHHC28II)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

601-411-1816

Syn­onyms

HHG-2

Squence

Amino Acid Sequence

IIGP­GRVVGS RRRP­PRK­LVP LAYKQF­SP­NV PEK­TL­GAS­GR YEGKIARSSE RFKELTP­NYN PDI­IFKDEEN TGADRLMTQR CKDRLNSLAI SVM­N­QW­PGVK LRVTEGWD­ED GHH­SEESL­HY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVK­SEHSA AAK­TGG

Syn­onyms

HHG-2

Acces­sion

Q14623

Gene ID

3549

Sum­ma­ry

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 19.8 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 176 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by its abil­i­ty to induce alka­line phos­phatase pro­duc­tion by C3H10T1/2(CCL-226) cells is 3.0-10 ug/​ml.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in 1 × PBS, pH 7.4.

Endo­tox­in

Less than 1 EU/​ug of rHuI­HH C28II as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

1. Kumar S, Bal­czarek KA, Lai ZC. 1996. Genet­ics. 142:965-72.2. Mari­go V, Roberts DJ, Lee SM, et al. 1995. Genomics. 28:44-51.3. Vortkamp A, Lee K, Lanske B, et al. 1996. Sci­ence. 273:613-22.

Puri­ty

> 96 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

5ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Human Indi­an Hedge­hog Cys28IleIle (rHuIHHC28II) on ChemWhat

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