Recom­bi­nant Human Ker­atinocyte Growth Fac­tor-2/FGF-10 (rHuKGF-2/FGF-10) CAS 104-10-1816

Offi­cial Full Name

Recom­bi­nant Human Ker­atinocyte Growth Fac­­tor-2/FGF-10 (rHuKGF-2/FGF-10)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

104-10-1816

Syn­onyms

Squence

Amino Acid Sequence

LGQD­MVS­PEA TNSSSSS­F­SS PSSAGRHVRS YNHLQGDVRW RKLFS­FTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAIN­SN YYLAMNKKGK LYGSKEFN­ND CKLK­ERIEEN GYNTYASFNW QHN­GRQMY­VA LNGK­GAPRRG QKTR­RKNTSA HFLP­MVVHS

Syn­onyms

Acces­sion

O15520

Gene ID

2255

Sum­ma­ry

Source

Mol­e­c­u­lar Weight

Approx­i­mate­ly 19.1 kDa, a sin­gle, non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 169 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by thymi­dine uptake assay using FGF-recep­­tors trans­fect­ed BaF3 cells is less than 0.5 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 2.0 × 106 IU/​mg.

Appear­ance

Ster­ile Fil­tered White lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in 2 × PBS, pH 7.4.

Endo­tox­in

Less than 1 EU/​ug of rHuKGF-2/FGF-10 as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

1. Emo­to H, Tagashira S, Mat­tei MG, et al. 1997. J Biol Chem. 272:23191-4.2. Tagashira S, Hara­da H, Kat­suma­ta T, et al. 1997. Gene. 197:399-404.3. Carn­in­ci P, Kasukawa T, Kataya­ma S, et al. 2005. Sci­ence. 309:1559-63.4. Igarashi M, Finch PW, Aaron­son SA. 1998. J Biol Chem. 273:13230-5.5. Ente­sar­i­an M, Mats­son H, Klar J, et al. 2005. Nat Genet. 37:125-7.

Puri­ty

> 97 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

5ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Human Ker­atinocyte Growth Fac­­tor-2/FGF-10 (rHuKGF-2/FGF-10) on ChemWhat

Quick Inquiry

Fill out our inquiry form and one of our experts will be in touch with you shortly.