Official Full Name
Recombinant Human Mucosae-associated Epithelial Chemokine/CCL28 (rHuMEC/CCL28)
CAS Number (Or Watson Number for Non-CAS Products)
204-28-1816
Synonyms
CCK1 Squence
Amino Acid Sequence
SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY Synonyms
CCK1 Accession
Q9NRJ3
Gene ID
56477
Summary
CCL28 belongs to
CC family chemokine, also named mucosae-associated epithelial chemokine (
MEC). The
CCL28 cDNA is located on Chr.5 in humans, and the mRNA of
CCL28 is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse
CCL28 shares 83 % identity, when the protein is processed to be the mature form.
CCL28 is signaling through
CCR3 and
CCR10, and chemotactic for resting
CD4,
CD8 T-cells and eosinophils.
Source
Escherichia coli.
Molecular Weight
Approximately 12.4 kDa, a single non-glycosylated polypeptide chain containing 108 amino acids.
Biological Activity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 1.0-10.0 ng/ml.
Appearance
Sterile filtered white lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2 um filtered concentrated solution in 20 mM
PB, pH 7.4, 130 mM NaCl.
Endotoxin
Less than 1
EU/ug of rHuMEC/CCL28 as determined by
LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 %
BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
References
Purity
> 97 % by
SDS-PAGE and
HPLC analyses.
SDS-PAGE
Pack Size
5ug/100ug/500ug
Safety Data Sheet (SDS) Download
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Technical Data Sheet (TDS) Download
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Links
This product is branded by ChemWhat and sold through our website and here is the corresponding link
Recombinant Human Mucosae-associated Epithelial Chemokine/CCL28 (rHuMEC/CCL28) on ChemWhat
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