Recom­bi­nant Murine Mid­kine (rMu­Mid­kine) CAS 127-18-1816

Offi­cial Full Name

Recom­bi­nant Murine Mid­kine (rMu­Mid­kine)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

127-18-1816

Syn­onyms

Retinoic Acid-induced Dif­fer­en­ti­a­tion Fac­tor

Squence

Amino Acid Sequence

VAKKKEKVKK GSEC­SEWTWG PCTPSSKD­CG MGFREGTC­GA QTQRVHCK­VP CNWKKE­F­GAD CKYK­FESWGA CDGST­GTKAR QGTLKKARYN AQC­QE­TIRVT KPCT­SK­TK­SK TKAKKGKGKD

Syn­onyms

Retinoic Acid-induced Dif­fer­en­ti­a­tion Fac­tor

Acces­sion

P12025

Gene ID

17242

Sum­ma­ry

Mid­kine, also named MK, MK1, NEGF 2, is belong­ing to the neu­rotroph­ic and devel­op­­men­­tal­­ly-reg­u­lat­ed heparin-bind­ing mol­e­cules fam­i­ly. It is encod­ed by the MDK gene. The Mid­kine pro­tein includes five intra­chain disul­fide bonds which hold two domains and there are three antipar­al­lel beta-sheets in each domain. A chon­droitin sul­fate pro­teo­gly­can, pro­tein-tyro­­sine phos­phatase zeta (PTPze­ta), is a recep­tor for MK. MK pro­motes the growth, sur­vival, and migra­tion of var­i­ous cells, and plays roles in neu­ro­ge­n­e­sis and epithe­lial mes­enchy­mal inter­ac­tions dur­ing organo­gen­e­sis. The pre­dict­ed mol­e­c­u­lar weight is approx­i­mate­ly 13.3 kDa, based on a mature pep­tide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Across species, MK shows 87 % iden­ti­ty between the human and murine pro­teins.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 13.2 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 120 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The bio­log­i­cal activ­i­ty deter­mined by a chemo­taxis bioas­say using human neu­trophils is in a con­cen­tra­tion range of 10-100 ng/​ml.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in 2 × PBS, pH7.4.

Endo­tox­in

Less than 1 EU/​ug of rMu­Mid­kine as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​ml. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20°C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

Puri­ty

> 96 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

5ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Murine Mid­kine (rMu­Mid­kine) on ChemWhat

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