Recom­bi­nant Rat Inter­leukin-2 (rRtIL-2) CAS 141-02-1816

Offi­cial Full Name

Recom­bi­nant Rat Inter­leukin-2 (rRtIL-2)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

141-02-1816

Syn­onyms

T-cell Growth Fac­tor, CGF

Squence

Amino Acid Sequence

APTSS­PAKET QQH­LE­QL­LLD LQVLL­RGIDN YKN­LKLP­MML TFK­FYLP­KQA TELKHLQ­CLE NEL­GALQRVL DLTQSKSFHL EDAG­N­FIS­NI RVTVVK­LKGS ENK­FEC­QFDD EPATVVE­FLR RWIAIC­QSII STMTQ

Syn­onyms

T-cell Growth Fac­tor, CGF

Acces­sion

P17108

Gene ID

116562

Sum­ma­ry

IL-2 is a pow­er­ful immunoreg­u­la­to­ry lym­phokine pro­duced by T-cells in response to anti­genic or mito­genic stim­u­la­tion. It is expressed by CD4+ and CD8+ T cells, gam­madelta T cells, B cells, den­drit­ic cells, and eosinophils. IL-2/IL-2R sig­nal­ing is required for T-cell pro­lif­er­a­tion and oth­er fun­da­men­tal func­tions which are essen­tial for the immune response. The recep­tor for IL-2 con­sists of three sub­units (55 kDa IL2Ralpha, 75 kDa IL2Rβ, 64 kDa com­mon gam­ma chain gammac/​IL2Rgamma) that are present on the cell sur­face in vary­ing pre­formed com­plex­es. Recom­bi­nant rat IL-2 is a 15.3 kDa pro­tein con­tain­ing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence iden­ti­ty with mouse, human, rhe­sus macaque, pig and equ­us cabal­lus IL-2, respec­tive­ly.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 15.5 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 135 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by a cell pro­lif­er­a­tion assay using murine CTLL-2 cells is less than 0.2 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 5.0 × 106 IU/​mg.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

A 0.2 um fil­tered con­cen­trat­ed ster­ile solu­tion in 10 mM sodi­um cit­rate, pH 4.0.

Endo­tox­in

Less than 1 EU/​ug of rRtIL-2 as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

Puri­ty

> 97 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

5ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Rat Inter­leukin-2 (rRtIL-2) on ChemWhat

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