Recom­bi­nant Rhe­sus Macaque Inter­leukin-4 (rRhIL-4) CAS 111-04-1816

Offi­cial Full Name

Recom­bi­nant Rhe­sus Macaque Inter­leukin-4 (rRhIL-4)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

111-04-1816

Syn­onyms

BSF-1, Lym­pho­cyte Stim­u­la­to­ry Fac­tor 1 

Squence

Amino Acid Sequence

HNCHIAL­REI IETLNSLTEQ KTLCTKLTIT DILAASKNTT EKET­FCRAAT VLRQ­FYSH­HE KDTR­CL­GA­TA QQFHRHKQLI RFLKRL­DRNL WGLAGLNSCP VKEAN­QS­TLE DFLER­LK­TIM REKYSKC­SS

Syn­onyms

BSF-1, Lym­pho­cyte Stim­u­la­to­ry Fac­tor 1 

Acces­sion

P51492

Gene ID

Sum­ma­ry

Inter­leukin-4 (IL-4) is a pleiotrop­ic cytokine main­ly pro­duced by mast cells, acti­vat­ed T cells and bone mar­row stro­mal cells. It has many bio­log­i­cal roles, includ­ing the stim­u­la­tion of acti­vat­ed B-cell and T-cell pro­lif­er­a­tion, and the dif­fer­en­ti­a­tion of CD4+ T-cells into Th2 cells. Addi­tion­al­ly, It enhances both secre­tion and cell sur­face expres­sion of IgE and IgG1 and also reg­u­lates the expres­sion of the low affin­i­ty Fc recep­tor for IgE (CD23) on both lym­pho­cytes and mono­cytes. The rhe­sus macaque IL-4 shares about 40 % ~ 45 % a.a. sequence iden­ti­ty with human, mouse, rat IL-4 and they are species-spe­­cif­ic in their activ­i­ties.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 14.9 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 129 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by a cell pro­lif­er­a­tion assay using human TF-1 cells is less than 1.0 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 1.0 × 106 IU/​mg.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in 2 × PBS, pH 7.4, 5 % tre­halose.

Endo­tox­in

Less than 1 EU/​ug of rRhIL-4 as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​ml. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20°C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

Puri­ty

> 96 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

5ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Rhe­sus Macaque Inter­leukin-4 (rRhIL-4) on ChemWhat

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