- Overview
- Specifications
- Shipping, Reconstitution & Storage
- General View of Documents
- Declaration
- Quick Inquiry
Overview
General Description
Recombinant SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) Protein is expressed from HEK293 with His tag at the C-terminal.It contains Gln14-Arg683(T19I, L24S, PPA25-27del, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H).
Biotinylated
Specifications
Target
Spike S1 (Omicron BA.4/BA.5/BA.5.2)
Synonyms
Accession
Species
SARS-COV-2
Exact Sequence
Gln14-Arg683(T19I, L24S, PPA25-27del, HV69-70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H)
Molecular Weight
The protein has a predicted MW of 75.76 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.
Activity
Immobilized SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) , His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 53.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Immobilized SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) , His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 53.4ng/ml determined by ELISA.
The purity of SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) is greater than 95% as determined by SEC-HPLC.
SARS-CoV-2 Spike S1 (Omicron BA.4/BA.5/BA.5.2) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
Quick Inquiry
Fill out our inquiry form and one of our experts will be in touch with you shortly.
