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- Specifications
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Overview
General Description
Recombinant Biotinylated Human FGL1 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Asp64-Asn305.
Biotinylated
Yes
Specifications
Target
FGL1
Synonyms
FGL1 ; FGL-1 ; Hepassocin ; HP-041 ; HFREP-1 ; LFIRE-1 ; HFREP1 ; Lag3 ligand
Accession
Species
Human
Exact Sequence
Asp64-Asn305
Molecular Weight
The protein has a predicted MW of 31.9 kDa same as Tris-Bis PAGE result.
Activity
Immobilized Human LAG3, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, His Tag with the EC50 of 0.48ug/ml determined by ELISA. See testing image for detail.
Product Tag
N-His-Avi
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Immobilized Human LAG3, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGL1, His Tag with the EC50 of 0.48ug/ml determined by ELISA.
Biotinylated Human FGL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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