Biotinylated Mouse GDF15 Protein (Primary Amine Labeling), Accession: Q9Z0J7 Watson International Limited

Overview
Specifications
Shipping, Reconstitution & Storage
General View of Documents
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Overview

General Description

Recombinant Biotinylated Mouse GDF15 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-terminal.It contains Ser189-Ala303.

Biotinylated

Yes

Specifications

Target

GDF15

Synonyms

GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta

Accession

Q9Z0J7

Species

Mouse

Exact Sequence

Ser189-Ala303

Molecular Weight

The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Mouse GFRAL, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GDF15, hFc Tag with the EC50 of 10ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-hFc

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

General View of Documents

Immobilized Mouse GFRAL, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GDF15, hFc Tag with the EC50 of 10ng/ml determined by ELISA.