- Overview
- Specifications
- Shipping, Reconstitution & Storage
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Overview
General Description
Recombinant Biotinylated Mouse GDF15 Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the N-terminal.It contains Ser189-Ala303.
Biotinylated
Yes
Specifications
Target
GDF15
Synonyms
GDF-15 ; MIC-1 ; NAG-1 ; PDF ; PLAB ; PTGFB ; GDF15 ; MIC1 ; RG-1 ; Placental TGF-beta ; PTGF-beta ; PTGFBPTGF-beta
Accession
Species
Mouse
Exact Sequence
Ser189-Ala303
Molecular Weight
The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Mouse GFRAL, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GDF15, hFc Tag with the EC50 of 10ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-hFc
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Immobilized Mouse GFRAL, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse GDF15, hFc Tag with the EC50 of 10ng/ml determined by ELISA.
The purity of Biotinylated Mouse GDF15 is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse GDF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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