Human GM-CSF Pro­tein, Acces­sion : P04141

• Overview
• Spec­i­fi­ca­tions
• Ship­ping, Recon­sti­tu­tion & Storage
• Gen­er­al View of Documents
• Dec­la­ra­tion
• Quick Inquiry

Overview

Gen­er­al Description

Recom­bi­nant Human GM-CSF Pro­tein is expressed from E.coli with­out tag​.It con­tains Ala18-Glu144.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

GM-CSF

Syn­onyms

CSF ; CSF2 ; GMCSF ; GM-CSF ; MGC131935 ; MGC138897

Acces­sion

P04141

Species

Human

Exact Sequence

Ala18-Glu144

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 14.6 kDa same as Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Human GM-CSF at 2ug/​ml (100ul/​Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml deter­mined by ELISA (QC Test). The affin­i­ty con­stant of 0.79nM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

No Tag

Express Sys­tem

E.coli

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 5% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Human GM-CSF R alpha, His Tag cap­tured on CM5 Chip via anti-His anti­body can bind Human GM-CSF, No Tag with an affin­i­ty con­stant of 0.79nM as deter­mined in SPR assay (Bia­core T200).

Immo­bi­lized Human GM-CSF at 2ug/​ml (100ul/​Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 16.9ng/ml deter­mined by ELISA (QC Test).

The ED50 as deter­mined by the dose-depen­­dent stim­u­la­tion of the pro­lif­er­a­tion of human TF-1 cells is 0.02-0.1 ng/​mL (QC Test).

The puri­ty of Human GM-CSF is greater than 95% as deter­mined by SEC-HPLC.

Human GM-CSF on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

Dec­la­ra­tion

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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