SARS-COV-2 Spike S1 (Deltacron) Pro­tein, Acces­sion : QHD43416

• Overview
• Spec­i­fi­ca­tions
• Ship­ping, Recon­sti­tu­tion & Storage
• Gen­er­al View of Documents
• Dec­la­ra­tion
• Quick Inquiry

Overview

Gen­er­al Description

Recom­bi­nant SARS-COV-2 Spike S1(Deltacron) Pro­tein is expressed from HEK293 with His tag at the C​-ter​mi​nal​.It con­tains Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H). Ala­nine sub­sti­tu­tions (R683A) are intro­duced to sta­bi­lize the trimer­ic pre­fu­sion state of SARS-COV-2 Spike S1(Deltacron) Pro­tein and abol­ish the furin cleav­age site, respectively.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

Spike S1 (Deltacron)

Syn­onyms

Acces­sion

QHD43416

Species

SARS-COV-2

Exact Sequence

Gln14-Arg683(T19R, A27S, T95I, G142D, EFR156G, NL211I, INS214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 73.94 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 90-120 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized SARS-COV-2 Spike S1 (Deltacron) , His Tag at 1ug/​ml (100ul/​Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 65.9ng/ml deter­mined by ELISA. See test­ing image for detail.

Prod­uct Tag

C-His

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Liq­uid

Ship­ping Conditions

Shipped with blue ice.

For­mu­la­tion

Sup­plied as 0.22um fil­tered solu­tion in PBS (pH 7.4).

Recon­sti­tu­tion

Sta­bil­i­ty & Storage

Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the pro­tein into small­er quan­ti­ties for opti­mal stor­age. Please avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Immo­bi­lized SARS-COV-2 Spike S1 (Deltacron) , His Tag at 1ug/​ml (100ul/​Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 65.9ng/ml deter­mined by ELISA.

SARS-COV-2 Spike S1 (Deltacron) on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

Dec­la­ra­tion

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

Quick Inquiry

Fill out our inquiry form and one of our experts will be in touch with you shortly.