- Overview
- Specifications
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Overview
General Description
Recombinant Biotinylated Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (Primary Amine Labeling) is expressed from HEK293 with hFc tag at the C-terminal.It contains Gln22-Asp117(CD3E)&Gln23-Thr113(CD3G).
Biotinylated
Yes
Specifications
Target
CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms
CD3 ; CD3G ; CD3g ; T3G ; CD3e ; CD3E ; CD3 epsilon&CD3 gamma ; CD3E&CD3G
Accession
Species
Cynomolgus
Exact Sequence
Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)
Molecular Weight
The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation,the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3G, hFc Tag with the EC50 of 83.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Immobilized Anti-CD3 Antibody, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus CD3E&CD3G, hFc Tag with the EC50 of 83.8ng/ml determined by ELISA.
The purity of Biotinylated Cynomolgus CD3E&CD3G was greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus CD3E&CD3G on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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