- Overview
- Specifications
- Shipping, Reconstitution & Storage
- General View of Documents
- Declaration
- Quick Inquiry
Overview
General Description
Recombinant Biotinylated Human AXL Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Ala26-Pro449.
Biotinylated
Yes
Specifications
Target
AXL
Synonyms
Axl ; UFO ; AXL oncogene ; AXL ; ARK ; JTK11 ; Tyro7 ; AI323647 ; EC 2.7.10 ; EC 2.7.10.1
Accession
Species
Human
Exact Sequence
Ala26-Pro449
Molecular Weight
The protein has a predicted MW of 48.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Anti-AXL Anitibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human AXL, His Tag with the EC50 of 0.87ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Immobilized Anti-AXL Anitibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human AXL, His Tag with the EC50 of 0.87ug/ml determined by ELISA.
The purity of Biotinylated Human AXL is greater than 95% as determined by SEC-HPLC.
Biotinylated Human AXL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
Quick Inquiry
Fill out our inquiry form and one of our experts will be in touch with you shortly.