Overview

Gen­er­al Description

Recom­bi­nant Biotiny­lat­ed Human Mid­kine Pro­tein is expressed from E.coli with His tag and Avi Tag at the N​-ter​mi​nal​.It con­tains Val21-Asp143.

Biotiny­lat­ed

Yes

Spec­i­fi­ca­tions

Tar­get

Mid­kine

Syn­onyms

MK ; Amphireg­ulin-asso­­ci­at­ed pro­tein ; ARAP ; Midges­ta­tion and kid­ney pro­tein ; Neu­rite out­­­growth-pro­­mot­ing fac­tor 2 ; Neu­rite out­­­growth-pro­­mot­ing pro­tein ; MDK ; MEK ; MK1 ; MKARAP ; NEGF2

Acces­sion

P21741

Species

Human

Exact Sequence

Val21-Asp143

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 16.46 kDa same as Tris-Bis PAGE result.

Activ­i­ty

Prod­uct Tag

N-His-Avi

Express Sys­tem

E.coli

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 8% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Biotiny­lat­ed Human Mid­kine on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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