Biotinylated Human NKG2A&CD94 Protein, Accession: P26715 Watson International Limited

Overview
Specifications
Shipping, Reconstitution & Storage
General View of Documents
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Overview

General Description

Recombinant Biotinylated Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-terminal.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).

Biotinylated

Yes

Specifications

Target

NKG2A&CD94

Synonyms

CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94

Accession

P26715

Species

Human

Exact Sequence

Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)

Molecular Weight

The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Anti-NKG2A Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human NKG2A&CD94, His Tag with the EC50 of 0.11ug/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Avi

Express System

HEK293

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

General View of Documents

Immobilized Anti-NKG2A Antibody, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human NKG2A&CD94, His Tag with the EC50 of 0.11ug/ml determined by ELISA.