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Overview
General Description
Recombinant Cynomolgus SIRP alpha/CD172a Protein is expressed from HEK293 with His tag at the C-terminal.It contains Glu31-Arg370.
Biotinylated
Specifications
Target
SIRP alpha/CD172a
Synonyms
CD172a ; BIT ; MFR ; MYD1 ; MYD-1 ; P84 ; PTPNS1 ; SHP substrate 1 ; SHPS1 ; SHPS-1 ; SHPS1CD172A ; SIRP alpha ; SIRPA ; Sirp-alpha-1 ; SIRPalpha2 ; Sirp-alpha-2 ; Sirp-alpha-3
Accession
Species
Cynomolgus
Exact Sequence
Glu31-Arg370
Molecular Weight
The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Cynomolgus SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA (QC Test). The affinity constant of 63.27 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Cynomolgus SIRP alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus /Rhesus macaque CD47, His Tag with an affinity constant of 63.27 nM as determined in SPR assay (Biacore T200).
Immobilized Cynomolgus SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA (QC Test).
The purity of Cynomolgus SIRP alpha is greater than 95% as determined by SEC-HPLC.
Cynomolgus SIRP alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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