Iden­ti­fi­ca­tion

CAS Number

62931-20-8

Name

D-xylose dehy­dro­ge­nase

Syn­onyms

CvXylB

Mol­e­c­u­lar weight

27kDa

Prop­er­ties

Appear­ance

Col­or­less clear to very slight­ly hazy liquid

Safe­ty Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our 

Prod­uct Description

CvXylB (Xylose Dehy­dro­ge­nase), EC : 1.1.1.175, is derived from Caulobac­ter vib­ri­oides and par­tic­i­pates in the degra­da­tion of D-xylose — a pri­ma­ry con­stituent of hemi­cel­lu­los­es such as xylan. It cat­alyzes the ini­tial reac­tion of the xylose uti­liza­tion path­way by oxi­diz­ing D-xylose into D-xylono-1,4-lactone. It exhibits some activ­i­ty toward L-ara­bi­nose but demon­strates neg­li­gi­ble activ­i­ty toward D-ara­bi­nose, L-xylose, D-ribose, D-galac­­tose, D-glu­­cose, or D-glucose-6-phosphate.

Unit Def­i­n­i­tion

One unit is defined as the amount of enzyme that cat­alyzes the for­ma­tion of 1 μmol of D-xylose-1,5-lactone from D-xylose at 37°C and pH 9.0.

Iden­ti­fi­ca­tion Methods

HNMR, HPLC

Puri­ty

＞90%

Enzyme activ­i­ty

≥1U/​mg

Buffer

20mMTris-HCl, pH7.5, 200mM NaCl, 10%glycerol

Sug­gest­ed Procedure

  1. Add the sub­strate to the reac­tion sys­tem ; option­al­ly, add a buffer salt (e.g., Tris-HCl) to adjust the pH to 9.0, fol­lowed by the addi­tion of an appro­pri­ate amount of CvXylB (based on reac­tion requirements).
  2. After mix­ing, incu­bate the sam­ple at 37°C for 1 – 2 hours.
    [Note] When scal­ing up the reac­tion sys­tem, the incu­ba­tion time or enzyme dosage should be adjust­ed accord­ing to actu­al con­di­tions ; low-speed shak­ing may be used to facil­i­tate mixing.

Stor­age

Trans­port with ice packs or dry ice ; store at -20°C ;

Shelf Life

18 months.

This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd (https://​www​.wat​son​-bio​.com/).

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