Recombinant expressed by E.coli
≥160U/mg yellowish amorphous lyophilized power. Refer to the COA and the bottle logo, or according to customer’s special requirement.
~26kDa by SDS-PAGE
Pale yellow amorphous lyophilized powder
NONH for all modes of transport
Specifications and Other Information of Our Diaphorase CAS UENA-0247
＞90% by SDS-PAGE (Fig 1).
One unit is defined as the amount of enzyme which oxidizes one micromole NADH per minute under the conditions of pH7.0 at 37℃ as described.The activity concentration (U/L) is calculated by detecting ∆A/min of formazane dye production rate at 550nm optical wavelength. Millimolar extinction coefficient of formazane dye under the assay conditions is 12.4 cm2/micromole.
1) Inhibitor : Sodium azide can be used as a preservative for enzyme solution and lyophilized powder without affecting activity and stability, but 0.1% sodium azide in the detection Reagent R1 and Reagent R2 can inhibit the activity about 25%.
2) Stabilizer and cofactor : FMN.
3) Optimum pH : pH7.0 (Fig 3).
4) pH stability : pH5.0~10.0 (Fig 4).
5) Optimum temperature : above 60℃ (Fig 5).
6) Thermal stability : below 70℃ in PBS, pH7.4 (Fig 6).
7) Freeze-Thaw Stability : after 20 freeze-thaw cycles, the activity of liquid was stable (Fig 7).
8) Accelerated stability : The activity of enzyme solution and lyophilized power are stable by incubated at 42℃ for 3 days and 37℃ for 14 days (Fig 8 and 9).
9) Special activity : ~400U/mg protein.
10) Km=0.22mM (NADH).
Stable at -20℃ for at least 12 months (Fig 2).
As an indicator enzyme, DPR is useful for enzymatic determination of D-3-Hydroxybutyrate when coupled with D-3-Hydroxybutyrate Dehydrogenase (HBDH, EC 220.127.116.11) in clinical analysis. DPR is also useful for enzymatic determination of NADH and other dehydrogenases.
This product is developed by our R&D company Ulcho Biochemical Ltd (http://www.ulcho.com/).
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