Human CCL5 Pro­tein, Acces­sion : P13501

• Overview
• Spec­i­fi­ca­tions
• Ship­ping, Recon­sti­tu­tion & Storage
• Gen­er­al View of Documents
• Dec­la­ra­tion
• Quick Inquiry

Overview

Gen­er­al Description

Recom­bi­nant Human CCL5 Pro­tein is expressed from E.coli with His-SUMO tag at the N​-ter​mi​nal​.It con­tains Ser24-Ser91.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

CCL5

Syn­onyms

MuRantes ; SIS-delta ; Scya5 ; Ccl5 ; D17S136E ; eoCP ; RANTES ; SCYA5 ; SISd ; TCP228 ; CCL5

Acces­sion

P13501

Species

Human

Exact Sequence

Ser24-Ser91

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 21 kDa. Due to the pro­tein can cleaved into two chains, so it migrates to 15-18 kDa and 22-25 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Human CCL5, His Tag at 0.5ug/ml (100ul/​well) on the plate. Dose response curve for Anti-CCL5 Anti­body, hFc Tag with the EC50 of 4.4ng/ml deter­mined by ELISA (QC Test). See test­ing image for detail.

Prod­uct Tag

N-His,SUMO

Express Sys­tem

E.coli

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in 20mM Tris,0.3M NaCl (pH 8.5). Nor­mal­ly 8% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Immo­bi­lized Human CCL5, His Tag at 0.5ug/ml (100ul/​well) on the plate. Dose response curve for Anti-CCL5 Anti­body, hFc Tag with the EC50 of 4.4ng/ml deter­mined by ELISA (QC Test).

Immo­bi­lized Human CCL5, His Tag at 5ug/​ml (100ul/​well) on the plate. Dose response curve for Biotiny­lat­ed Human CXCL4, His Tag with the EC50 of 1.1ug/ml deter­mined by ELISA.

Human CCL5 on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

Dec­la­ra­tion

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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