- Overview
- Specifications
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Overview
General Description
Recombinant Human Her2/ErbB2 Domain 4 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Pro489-Cys630.
Biotinylated
Specifications
Target
Her2/ErbB2 Domain 4
Synonyms
CD340 ; EGFR2 ; ErbB2 ; HER2 ; HER-2 ; herstatin ; MLN 19 ; MLN19 ; Neu Oncogene ; NEUHER-2/neu ; NGL ; p185erbB2 ; TKR1 ; ERBB2 ; HER-2/neu ; NEU ; ENV ; ENVW ; ERVWE1 ; HERV-7q ; HERV-W-ENV ; HERV7Q ; HERVW ; HERVWENV
Accession
Species
Human
Exact Sequence
Pro489-Cys630
Molecular Weight
The protein has a predicted MW of 18.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Human Her2 Domain 4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 28.5ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Immobilized Human Her2 Domain 4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 28.5ng/ml determined by ELISA.
The purity of Human Her2 Domain 4 is greater than 95% as determined by SEC-HPLC.
Human Her2 Domain 4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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