Overview

Gen­er­al Description

Recom­bi­nant Human IL-2 R alpha/​CD25 Pro­tein is expressed from HEK293 with His tag and Avi tag at the C​-ter​mi​nal​.It con­tains Glu22-Cys213.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

IL-2 R alpha/​CD25

Syn­onyms

CD25 ; IDDM10 ; IL-2 R alpha ; IL2R ; IL2RA ; p55 ; TCGFR

Acces­sion

P01589

Species

Human

Exact Sequence

Glu22-Cys213

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 24.6 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Human IL-2 R alpha, His Tag at 0.2ug/ml (100ul/​well) on the plate. Dose response curve for Anti-IL-2 R alpha Anti­body, hFc Tag with the EC50 of 9.6ng/ml deter­mined by ELISA. The affin­i­ty con­stant of 15.2nM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

C-His-Avi

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 5% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Human IL-2 R alpha, His Tag cap­tured on CM5 Chip via anti-His anti­body can bind Human IL-2, No Tag with an affin­i­ty con­stant of 15.2nM as deter­mined in SPR assay (Bia­core T200).

Immo­bi­lized Human IL-2 R alpha, His Tag at 0.2ug/ml (100ul/​well) on the plate. Dose response curve for Anti-IL-2 R alpha Anti­body, hFc Tag with the EC50 of 9.6ng/ml deter­mined by ELISA.

The puri­ty of Human IL-2 R alpha is greater than 95% as deter­mined by SEC-HPLC.

Human IL-2 R alpha on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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