- Overview
- Specifications
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Overview
General Description
Recombinant Human KRAS G12D (HLA-A*11:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M) and VVVGADGVGK peptide.
Biotinylated
Specifications
Target
KRAS G12D (HLA-A*11:01)
Synonyms
MHC ; KRAS ; K-Ras 2 ; KRAS2 ; C-K-RAS ; CFC2 ; K-RAS2A ; K-RAS2B ; K-RAS4A ; K-RAS4B ; KRAS1 ; KRAS2 ; NS ; NS3 ; RASK2 ; GTPase Kras ; KI-RAS ; RALD
Accession
Species
Human
Exact Sequence
Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGADGVGK peptide
Molecular Weight
The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.
Activity
The affinity constant of 14.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Human KRAS G12D (HLA-A*11:01) , His Tag captured on CM5 Chip via Anti-His Antibody can bind KRAS G12D (HLA-A*11:01) (VVVGADGVGK) TCR with an affinity constant of 14.91 nM as determined in SPR assay (Biacore T200).
The purity of Human KRAS G12D (HLA-A*11:01) is greater than 95% as determined by SEC-HPLC.
Human KRAS G12D (HLA-A*11:01) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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