Human PLAU/​uPA Pro­tein (active form), Acces­sion : P00749

• Overview
• Spec­i­fi­ca­tions
• Ship­ping, Recon­sti­tu­tion & Storage
• Gen­er­al View of Documents
• Dec­la­ra­tion
• Quick Inquiry

Overview

Gen­er­al Description

Recom­bi­nant Human PLAU/​uPA Pro­tein (active form) is expressed from HEK293 with His tag at the C​-ter​mi​nal​.It con­tains Ser21-Leu431, which con­sists of two chains : Long chain A (Ser21-Phe177) and chain B (Ile179-Leu 431). The long chain A is fur­ther cleaved to yield a short chain A (Lys156-Phe 177) and N-ter­mi­­nal frag­ment (Ser21-Lys155).

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

PLAU/​uPA

Syn­onyms

PLAU ; Uroki­nase ; ATF ; UPA ; URK ; u-PA ; BDPLT5 ; QPD

Acces­sion

P00749

Species

Human

Exact Sequence

Ser21-Leu431

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 17.9 kDa (long chain A), 29.2 kDa (chain B) and 15.3 kDa (N-ter­mi­­nal frag­ment). The pro­tein migrates to 30-38 kDa (chain B) and 17 kDa (N-ter­mi­­nal frag­ment) based on Tris-Bis PAGE result.

Activ­i­ty

Prod­uct Tag

C-His

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in 25mM HEP­ES, 150mM NaCl (pH 7.5). Nor­mal­ly 8% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Mea­sured by its abil­i­ty to cleave a pep­tide sub­strate, N-car­boben­zy­loxy-Gly-Gly-Arg-7-ami­­do-4-methyl­­coumarin (Z-GGR-AMC). The spe­cif­ic activ­i­ty is > 2000 pmol/​min/​ug (QC Test).

The puri­ty of Human PLAU (acti­vat­ed by trypsin) is greater than 95% as deter­mined by SEC-HPLC.

Human PLAU (acti­vat­ed by trypsin) on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

Dec­la­ra­tion

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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