Human R spondin 3/RSPO3 Pro­tein, Acces­sion : Q9BXY4

• Overview
• Spec­i­fi­ca­tions
• Ship­ping, Recon­sti­tu­tion & Storage
• Gen­er­al View of Documents
• Dec­la­ra­tion
• Quick Inquiry

Overview

Gen­er­al Description

Recom­bi­nant Human R spondin 3/RSPO3 Pro­tein is expressed from HEK293 with His tag at the C​-ter​mi​nal​.It con­tains Gln22-Val146.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

R spondin 3/RSPO3

Syn­onyms

Acces­sion

Q9BXY4

Species

Human

Exact Sequence

Gln22-Val146

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 14.95 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 20-35 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Human R-Spondin 3, His Tag at 1ug/​ml (100ul/​well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml deter­mined by ELISA (QC Test). The affin­i­ty con­stant of 0.59 nM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

C-His

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Liq­uid

Ship­ping Conditions

Shipped with blue ice.

For­mu­la­tion

Sup­plied as 0.22um fil­tered solu­tion in PBS (pH 7.4).

Recon­sti­tu­tion

Sta­bil­i­ty & Storage

Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the pro­tein into small­er quan­ti­ties for opti­mal stor­age. Please avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Human LGR-5, hFc Tag cap­tured on CM5 Chip via Pro­tein A can bind Human R-Spondin 3, His Tag with an affin­i­ty con­stant of 0.59 nM as deter­mined in SPR assay (Bia­core T200) (Rou­tine Test).

Immo­bi­lized Human R-Spondin 3, His Tag at 1ug/​ml (100ul/​well) on the plate. Dose response curve for Human RNF43, hFc Tag with the EC50 of 25.2ng/ml deter­mined by ELISA (QC Test).

Human R spondin 3 on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

Dec­la­ra­tion

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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