Peni­cil­li­nase CAS 9001-74-5

• Iden­ti­fi­ca­tion
• Prop­er­ties
• Safe­ty Data
• Spec­i­fi­ca­tions & Oth­er Information
• Links
• Quick Inquiry

Iden­ti­fi­ca­tion

CAS Number

9001-74-5

Name

Peni­cil­li­nase

Prop­er­ties

Appear­ance

Col­or­less clear liquid

Safe­ty Data

Per­son­al Pro­tec­tive Equipment

dust mask type N95 (US), Eye­shields, Gloves

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our Peni­cil­li­nase CAS 9001-74-5

Descrip­tion

Peni­cil­li­nase, also known as β-lac­­ta­­mase, peni­cillin amino β-lac­­tam hydro­lase, is wide­ly dis­trib­uted in var­i­ous microor­gan­isms, espe­cial­ly in bacteria.The enzyme can effec­tive­ly hydrolyze the β-lac­­tam ring of β-lac­­tam antibi­otics, there­by los­ing the antimi­cro­bial activ­i­ty and hydrolyz­ing β-lac­­tam antibi­otics, includ­ing peni­cillin G, ampi­cillin, ampi­cillin, amox­i­cillin, and Ammo­nia and the like.
This prod­uct is iso­lat­ed and puri­fied from recom­bi­nant E. coli, and is ster­il­ized by fil­tra­tion to obtain a ster­ile peni­cil­li­nase solution.

Advan­tage

1. No ani­mal ori­gin : no ani­mal dis­eases, no path­o­gen­ic sub­stances, no con­t­a­m­i­na­tion by exoge­nous fac­tors, and high safety.
2. Qual­i­ty sta­bil­i­ty : This prod­uct is a lyophilized pow­der con­tain­ing a bio­log­i­cal­ly active pro­tec­tive agent. It has been mass-pro­­duced, and the dif­fer­ence between batch­es is small ; the pro­duc­tion scale is more than 1000L.

Prop­er­ties

Enzyme activ­i­ty definition

In phos­phate buffer at pH 7.0, 37 ℃ , the amount of enzyme required to con­vert 1 unit of peni­cillin in 1 hour is 1 U.

Prod­uct Usage

1.Sterility test and micro­bial lim­it test for β- lac­tam antibi­ot­ic drugs.
2.Removal of resid­ual peni­cillin in the envi­ron­ment and med­ical equip­ment.
3.Rapid quan­ti­ta­tive deter­mi­na­tion of peni­cillin residues in milk and treat­ment of peni­cillin excess milk.
4.New antibac­te­r­i­al drug screen­ing.
5.ELISA assay using peni­cil­li­nase as a label­ing enzyme.

Rec­om­mend­ed Method

1. Mem­brane fil­tra­tion method (see Chi­nese Phar­ma­copoeia 2020) 

(1) Take an appro­pri­ate amount of peni­cil­li­nase intothe water for injec­tion and con­fig­ure it as a rinse solu­tion. Ster­il­ize all the used equip­ment in a suit­able method and then soak it in the rinse solu­tion for 10 minutes.

(2) Take a spec­i­fied amount of the test sam­ple, fil­ter it accord­ing to the spec­i­fied method, and rinse the fil­ter with an appro­pri­ate amount of rinse solution. 

(3) Anoth­er appro­pri­ate amount of peni­cil­li­nase is added to the water for injec­tion to a cer­tain con­cen­tra­tion of the solu­tion, and an appro­pri­ate amount of the solu­tion is added to the medium. 

(4) The fil­ter is inoc­u­lat­ed into the medi­um accord­ing to a pre­scribed method. 

2. Direct inoc­u­la­tion method (see 2020 Chi­nese Pharmacopoeia) 

A pre­de­ter­mined amount of the test sam­ple is tak­en, mixed, and an appro­pri­ate amount of the ster­ile peni­cil­li­nase solu­tion is added, and inoc­u­lat­ed into each tube medium.Or direct­ly into the medi­um con­tain­ing ster­ile penicillinase.

Trans­port and Stor­age Stability

Trans­porta­tion sta­bil­i­ty : It is trans­port­ed in an ice pack to keep it active. 

Stor­age sta­bil­i­ty : 2~8 °C, pro­tect­ed from light, sta­ble stor­age for 8 months​.It should be used up once after open­ing the bottle.

Links

This prod­uct is devel­oped by our R&D com­pa­ny Apnoke Sci­en­tif­ic Ltd (http://​www​.apnoke​.com/).

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