Offi­cial Full Name

Recom­bi­nant Human Cu/​Zn Super­ox­ide Dis­mu­tase, His (rHuCu/ZnSOD,His)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

401-018-1816

Syn­onyms

SOD1

Squence

Squence of Recombinant Human Cu/Zn Superoxide Dismutase, His

Amino Acid Sequence

MGH­H­H­H­H­H­HH HHSS­GHIEGR HMT­YARAAAR QAR­ALEAT­KA VCVLKGDG­PV QGI­IN­FE­QKE SNG­PVKVWGS IKGLTEGL­HG FHVHE­FGDNT AGCT­SAG­PHF NPLSRKHG­GP KDEER­HVGDL GNVTAD­KDGV ADVSIEDSVI SLS­GDHCI­IG RTLVVHEKAD DLGKG­GNEES TKT­G­NAGSRL ACGVI­GIAQ

Syn­onyms

SOD1

Acces­sion

P00441

Gene ID

6647

Sum­ma­ry

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 39.9 kDa, a homod­imer, non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 2 × 189 amino acids with Met, Gly and 10 × His at N-ter­mi­nus.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The poten­cy per mg was deter­mined by Pyro­gal­lic Acid method and was found to be more than 1.0 × 104 IU/​mg.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in PBS, pH 7.4.

Endo­tox­in

Less than 1 EU/​ug of rHuCu/​Zn SOD, His as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in ster­ile dis­tilled water or aque­ous buffer con­tain­ing 0.1 % BSA to a con­cen­tra­tion of 0.1-1.0 mg/​mL. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

1. Kwia­tows­ki J, Skarecky D, Ayala FJ. 1992. Mol Phy­lo­genet Evol, 1 : 72-82.2. Bachus R, Claus A, Megow D, et al. 1995. J Neu­rol Sci, 129 Sup­pl : 93-5.3. Qi X, Guy J, Nick H, et al. 1997. Invest Oph­thal­mol Vis Sci, 38 : 1203-12.4. Chou CM, Huang CJ, Shih CM, et al. 2005. Ann N Y Acad Sci, 1042 : 303-13.5. Raja SB, Murali MR, Roopa K, et al. 2011. Bio­med Phar­ma­cother, 65 : 560-8.

Puri­ty

> 95 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

SDS-PAGE of Recombinant Human Cu/Zn Superoxide Dismutase, His

Pack Size

100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Human Cu/​Zn Super­ox­ide Dis­mu­tase, His (rHuCu/ZnSOD,His) on ChemWhat

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