Recombinant Human Insulin-like Growth factor-2 (rHuIGF-2) CAS 105-02-1816 Watson International Limited

Official Full Name

Recombinant Human Insulin-like Growth factor-2 (rHuIGF-2)

CAS Number (Or Watson Number for Non-CAS Products)

105-02-1816

Synonyms

Squence

region 105 02 1 - Recombinant Human Insulin-like Growth factor-2 (rHuIGF-2) CAS 105-02-1816

Amino Acid Sequence

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Synonyms

Accession

E3UN46

Gene ID

3481

Summary

Source

Molecular Weight

Approximately 7.5 kDa, a single non-glycosylated polypeptide chain containing 67 amino acids.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20.

Endotoxin

Less than 1 EU/ug of rHuIGF-2 as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.