Recom­bi­nant Rat Car­diotrophin-1 (rRtCT-1) CAS 641-30-1816

Offi­cial Full Name

Recom­bi­nant Rat Car­­diotrophin-1 (rRtCT-1)

CAS Num­ber (Or Wat­son Num­ber for Non-CAS Products)

641-30-1816

Syn­onyms

Squence

Amino Acid Sequence

MSQREGSLED HQTDSS­FS­FL PHLEAKIRQT HNLAR­LLTKY ADQLLEEYVQ QQGEPFGLPG FSP­PRLPLAG LSG­PAP­SHAG LPVSERL­RQD AAAL­SAL­PAL LDAVR­RRQAE LNPRAPRLLR SLEDAAR­QVR ALGAAVETVL AAL­GAAARGP VPEP­VAT­SAL FTSNSAAGVF SAKVL­GL­HVC GLYGEWVS­RT EGDL­GQLVPG GVA

Syn­onyms

Acces­sion

Q63086

Gene ID

29201

Sum­ma­ry

Cardiotrophin­1 (CT­1) is a mem­ber of the cytokine fam­i­ly which also includes IL-6, IL-11, leukemia inhibito­ry fac­tor (LIF), onco­statin M (OSM), and cil­iary neu­rotroph­ic fac­tor (CNTF). CT-1 is a pleiotrop­ic cytokine which is expressed in var­i­ous tis­sues includ­ing the adult heart, skele­tal mus­cle, ovary, colon, prostate and fetal lung. Stud­ies showed CT-1 which induces car­diac myocyte hyper­tro­phy in vit­ro can bind to and acti­vate the ILST/​gp130 recep­tor. Rat CT­1 encodes a 203 amino acid (a.a.) residue pro­tein that lacks a hydropho­bic sig­nal pep­tide and it shares 94 % a.a. and 79 % a.a. sequence iden­ti­ty with human and murine CT-1.

Source

Escherichia coli.

Mol­e­c­u­lar Weight

Approx­i­mate­ly 21.4 kDa, a sin­gle non-gly­­co­sy­lat­ed polypep­tide chain con­tain­ing 203 amino acids.

Bio­log­i­cal Activity

Ful­ly bio­log­i­cal­ly active when com­pared to stan­dard. The ED50 as deter­mined by a cell pro­lif­er­a­tion assay using human TF-1 cells is less than 0.5 ng/​ml, cor­re­spond­ing to a spe­cif­ic activ­i­ty of > 2.0 × 106 IU/​mg.

Appear­ance

Ster­ile fil­tered white lyophilized (freeze-dried) pow­der.

For­mu­la­tion

Lyophilized from a 0.2 um fil­tered con­cen­trat­ed solu­tion in 30 % Ace­toni­trile, 0.1 % TFA.

Endo­tox­in

Less than 1 EU/​ug of rRtCT-1 as deter­mined by LAL method.

Recon­sti­tu­tion

We rec­om­mend that this vial be briefly cen­trifuged pri­or to open­ing to bring the con­tents to the bot­tom. Recon­sti­tute in 4mM HCl to a con­cen­tra­tion of 0.1-1.0 mg/​ml. Stock solu­tions should be appor­tioned into work­ing aliquots and stored at ≤ -20 °C. Fur­ther dilu­tions should be made in appro­pri­ate buffered solu­tions.

Sta­bil­i­ty and Storage

Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as sup­plied.- 1 month, 2 to 8 °C under ster­ile con­di­tions after recon­sti­tu­tion.- 3 months, -20 to -70 °C under ster­ile con­di­tions after recon­sti­tu­tion.

Ref­er­ences

Puri­ty

> 95 % by SDS-PAGE and HPLC analy­ses.

SDS-PAGE

Pack Size

10ug/​100ug/​500ug

Safe­ty Data Sheet (SDS) Down­load

Click to download

Tech­ni­cal Data Sheet (TDS) Down­load

Click to download

Links

This prod­uct is brand­ed by ChemWhat and sold through our web­site and here is the cor­re­spond­ing link Recom­bi­nant Rat Car­­diotrophin-1 (rRtCT-1) on ChemWhat

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