Structure of RNase III CAS UENA 0194 - RNase III CAS UENA-0194

Iden­ti­fi­ca­tion

CAS Number

UENA-0194

EC Number

3.1.26.3

Name

RNase III

Prop­er­ties

Opti­mal reac­tion temperature

37℃

Def­i­n­i­tion of active unit

Def­i­n­i­tion of active unit : 1 active unit is defined as the amount of enzyme need­ed to degrade 1μg of dsR­NA into siR­NA in 20min at 37℃ in a 50μL reac­tion system.

Safe­ty Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our RNase III CAS UENA-0194

Prod­uct Introduction

This prod­uct is the RNase Ⅲ recom­bi­nant­ly expressed in E. coli. This spe­cif­ic exonu­cle­ase is capa­ble of cut­ting dou­ble-strand­ed RNA (dsR­NA) and gen­er­at­ing 12-35bp dsR­NA frag­ments with pro­trud­ing 5′-PO4, 3′-OH, and 3’ termini.

Prod­uct Features

Effi­cient pro­duc­tion of siR­NA ; accu­rate inter­fer­ence with tar­get gene expression.

Product Features 6 - RNase III CAS UENA-0194
Prod­uct Features

Prod­uct Composition

Item No.Com­po­si­tionStor­age Tem­per­a­ture
(℃ )
200U2000U
IRNase III (2U/​μL)-200.5mL5mL
10x Reac­tion Buffer-20 1.5mL15mL
10x EDTA-20 1mL10mL
10x MnCl2-20 1mL10mL

10× Reac­tion buffer con­tains 500mM Tris-HCl(pH7.5)500mM NaCl and 10mM DTT.

Qual­i­ty Control

Puri­ty ≥ 95%, resid­ual host cell DNA ≤ 100pg/​mg, resid­ual host cell pro­tein ≤ 50ppm, resid­ual endo­tox­in ≤10EU/​mg, no resid­ual RNase, endonu­cle­ase, exonu­cle­ase or pro­tease, germ-free, pathogen-free.

This prod­uct is devel­oped by our R&D com­pa­ny Ulcho Bio­chem­i­cal Ltd (http://​www​.ulcho​.com/).

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