T4 RNA lig­ase CAS UENA-0195

• Iden­ti­fi­ca­tion
• Prop­er­ties
• Safe­ty Data
• Spec­i­fi­ca­tions & Oth­er Information
• Links
• Quick Inquiry

Iden­ti­fi­ca­tion

CAS Number

UENA-0195

EC Number

6.5.1.3

Name

T4 RNA ligase

Prop­er­ties

Opti­mal reac­tion temperature

37℃

Def­i­n­i­tion of active unit

Def­i­n­i­tion of active unit : 1 active unit is defined as the amount of enzyme need­ed to con­vert 1nM 5’-[32P]rA16 into anti-phos­phor­ic acid form in 30min at 37℃ .

Safe­ty Data

RIDADR 

NONH for all modes of transport

WGK Germany

3

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our T4 RNA lig­ase CAS UENA-0195

Prod­uct Introduction

This prod­uct is the ATP-depen­­dent T4RNA Lig­ase I recom­bi­nant­ly expressed in E. coli. It can cat­alyze oligonu­cleotide, sin­­gle-strand­ed RNA and DNA intermolecular/​intramol­e­c­u­lar 5’-PO4 and 3’-OH to form phos­pho­di­ester bond.

Prod­uct Composition

1x Reac­tion buffer con­tains 50mM Tris-HCL (pH7.5), 10mM MgCl2 and 1mM DTT.

Qual­i­ty Control

Puri­ty ≥ 95%, resid­ual host cell DNA ≤ 100pg/​mg, resid­ual host cell pro­tein ≤ 50ppm, resid­ual endo­tox­in ≤10EU/​mg, no resid­ual RNase, endonu­cle­ase, exonu­cle­ase or pro­tease, germ-free, pathogen-free.
Prod­uct Usage

Prod­uct Usage

• RNA 3′ ter­mi­nus labelling (using cytidine-3′,5′-[-32P] diphos­phate)
• Con­nect­ing RNAs
• Syn­the­siz­ing olig­ori­bonu­cleotide and oligodeoxyri­bonu­cleotide
• Spe­cif­ic mod­i­fi­ca­tion of tRNA
• Con­nect­ing oligodeoxyri­bonu­cleotide to sin­­gle-strand­ed cDNA to real­ize 5’RACE (rapid ampli­fi­ca­tion of cDNA ter­mi­ni)
• Site-spe­­cif­ic gen­er­a­tion of mul­ti­plex PCR primer

Links

This prod­uct is devel­oped by our R&D com­pa­ny Ulcho Bio­chem­i­cal Ltd (http://​www​.ulcho​.com/).

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