Iden­ti­fi­ca­tion

CAS Number

None

Name

TsC­Dase

Mol­e­c­u­lar weight

72kD

EC Number

3.2.1.54

Prop­er­ties

Appear­ance

Col­or­less to light brown liquid

Safe­ty Data

RIDADR 

NONH for all modes of transport

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our TsC­Dase CAS# EC:3.2.1.54

Prod­uct Description

The TsC­Dase gene is derived from Ther­mo­coc­cus sp. Strain B1001 (EC : 3.2.1.54) and is recom­bi­nant­ly expressed in Escherichia coli.
It cat­alyzes the hydrol­y­sis of cyclodex­trins into mal­tose ; specif­i­cal­ly, α-cyclodex­trin is hydrolyzed into mal­tooc­taose, β-cyclodex­trin into mal­to­hep­taose, and γ-cyclodex­trin into maltohexaose.

Unit Def­i­n­i­tion

One unit is defined as the amount of enzyme that cat­alyzes the for­ma­tion of 1 μmol of (Glcα1,4)₆Glc from β-cyclodex­trin per minute at 80°C and pH 5.0.

Puri­ty

＞80%

Activ­i­ty

≥1000U/​ml

Sug­gest­ed Usage

  1. Add the sub­strate to the reac­tion sys­tem ; a buffer salt may be added as appro­pri­ate to adjust the pH to 5.0. Add a suit­able amount of TsC­Dase (based on reac­tion requirements).
  2. After mix­ing thor­ough­ly, incu­bate the sam­ple at 70°C for 2 – 4 hours.
  3. To ter­mi­nate the reac­tion, add 50% anhy­drous ethanol, mix by stir­ring, and then cen­trifuge and fil­ter the solu­tion.
    [Note] When scal­ing up the reac­tion sys­tem, the incu­ba­tion time or enzyme dosage should be adjust­ed accord­ing to actu­al con­di­tions ; low-speed shak­ing may be used for mixing.

Stor­age Buffer

20mM Tris-HCl, pH 7.5, 200mM NaCl,10% glycerol.

Shelf Life

2 years

Stor­age

-20°C with sealed well is 18 months.

This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd (https://​www​.wat​son​-bio​.com/).

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