Overview

Gen­er­al Description

The 7-methyl­guano­­sine cap struc­ture (m7Gppp, Cap0) is a spe­cif­ic fea­ture in eukary­ot­ic mRNA, which is required for mRNA sta­bil­i­ty, splic­ing, nuclear export, effec­tive trans­la­tion, and mRNA decay. Vac­cinia Cap­ping Enzyme, a het­erodimer of D1 and D12 sub­units, adding the Cap0 struc­ture to the 5′ end of the RNA. The D1 sub­unit com­pris­es an N-ter­mi­­nal RNA triphos­phatase (TPase), a guany­lyl­trans­ferase (GTase), and a C-ter­mi­­nal gua­­nine-N7-methyl­­tran­s­ferase (MTase). The D12 sub­unit binds and stim­u­lates the MTase. NOTE : mRNA Cap 2′-O-Methyltransferase (ChemWhat #GMP-MEH-VE101) is required to gen­er­ate Cap 1 struc­ture that reduces cel­lu­lar innate immune response when the RNA is used in vivo.

Biotiny­lat­ed

Spec­i­fi­ca­tions

Tar­get

Syn­onyms

Acces­sion

Species

Exact Sequence

Mol­e­c­u­lar Weight

Activ­i­ty

Prod­uct Tag

Express Sys­tem

Puri­ty

Endo­tox­in

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Liq­uid

Ship­ping Conditions

Shipped with blue ice

For­mu­la­tion

Recon­sti­tu­tion

Sta­bil­i­ty & Storage

-20±5°C for 24 months. Avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Analy­sis of mRNA cap­ping effi­cien­cy using LC-MS.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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