5(6)-Nitrofluorescein CAS 78512-32-0

• Iden­ti­fi­ca­tion
• Prop­er­ties
• Safe­ty Data
• Spec­i­fi­ca­tions & Oth­er Information
• Links
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Iden­ti­fi­ca­tion

CAS Number

78512-32-0

Name

5(6)-Nitrofluorescein

Syn­onyms

3′,6′-Dihydroxy-5-nitro-3H-spiro[2-benzofuran-1,9′-xanthen]-3-on
3′,6′-Dihydroxy-5-nitro-3H-spiro[2-benzofuran-1,9′-xanthen]-3-one
3′,6′-Dihydroxy-5-nitro-3H-spiro[2-benzofuran-1,9′-xanthen]-3-one[French][ACD/IUPAC Name]
3′,6′-dihydroxy-5-nitro-3H-spiro[isobenzofuran-1,9′-xanthen]-3-one
Spiro(isobenzofuran-1(3H),9′-(9H)xanthen)-3-one, 3′,6′-dihydroxy-5-nitro-
Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, 3′,6′-dihydroxy-5-nitro-
3′,6′-dihydroxy-5-nitrospiro[2-benzofuran-1,9′-xanthen]-3-one
3′,6′-DIHYDROXY-5-NITROSPIRO[2-BEN­ZO­FU­RAN-1,9′-XANTHENE]-3-ONE
3′,6′-dihydroxy-6-nitrospiro[2-benzofuran-3,9′-xanthene]-1-one
4-NITRO­FLU­O­RES­­CEIN
5-Nitro­flu­o­res­­cein
5-Nitro­flu­o­res­­cein (iso­mer I)
MFCD00059693[MDL num­ber]
MFCD00059694[MDL num­ber]
MFCD00135108[MDL num­ber]
Nitro­flu­o­res­cein, iso­mer 1
Spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one,3′,6′-dihydroxy-5-nitro-

SMILES

O=C1OC2(c3ccc(O)cc3Oc3cc(O)ccc32)c2ccc(N+[O-])cc21

Std­InChI

InChI=1S/C20H11NO7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)20(15)14-4-1-10(21(25)26)7-13(14)19(24)28-20/h1-9,22-23H

Std­InChIKey

UACQOMKZFGN­R­BL-UHF­F­­FAOYSA-N

Mol­e­c­u­lar Formula

C20H11NO7

Mol­e­c­u­lar Weight

377.308

MDL Number

MFCD00059693

Prop­er­ties

Appear­ance

Yel­low solid

Safe­ty Data

RIDADR 

NONH for all modes of transport

Spec­i­fi­ca­tions and Oth­er Infor­ma­tion of Our 5(6)-Nitrofluorescein CAS 78512-32-0

Iden­ti­fi­ca­tion Methods

HNMR, HPLC

Puri­ty

97% min

Shelf Life

1 year

Stor­age

Under room tem­per­a­ture away from light

Known Appli­ca­tion

1. Flu­o­res­cent probe devel­op­ment
   Due to its adjustable flu­o­res­cence response at spe­cif­ic wave­lengths, 5(6)-Nitrofluorescein is often used to devel­op flu­o­res­cent probes and mol­e­c­u­lar mark­ers, espe­cial­ly in bioimag­ing or ana­lyt­i­cal chem­istry. Nitro sub­sti­tu­tion will quench flu­o­res­cence and can be used to construct ​“on/​off” probes (such as indi­ca­tors for enzyme reac­tion mon­i­tor­ing or redox reactions).
2. Bio­la­bel­ing / cova­lent cou­pling reac­tion
   It can react with amino com­pounds through its car­boxyl group to form a sta­ble cova­lent bond, which is used for label­ing and track­ing bio­log­i­cal macro­mol­e­cules such as pro­teins and nucle­ic acids. It is used in con­junc­tion with flu­o­res­cence microscopy, flow cytom­e­try, etc.
3. Spec­tro­scop­ic research
   It is used as a mod­el com­pound to study spec­tral prop­er­ties such as inter­mol­e­c­u­lar ener­gy trans­fer, elec­tron­ic effects, and changes in flu­o­res­cence quan­tum yield.
4. Used to syn­the­size more com­plex flu­o­res­cent dyes or sen­sors
   It is an inter­me­di­ate or start­ing mate­r­i­al for many flu­o­res­cent deriv­a­tives (such as FRET probes, reac­tive oxy­gen detec­tion dyes, etc.).

Gen­er­al View of Documents

Links

This prod­uct is devel­oped by our R&D com­pa­ny Wat­son Bio Ltd (https://​www​.wat​son​-bio​.com/).

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