Overview

General Description

Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed from E.coli without tag.It contains Tyr37-Asp193.

Biotinylated

Yes

Specifications

Target

IL-18

Synonyms

IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1

Accession

Q14116

Species

Human

Exact Sequence

Tyr37-Asp193

Molecular Weight

The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result.

Activity

Immobilized Human IL-18BP, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 26.0ng/ml determined by ELISA. See testing image for detail.

Product Tag

No Tag

Express System

E.coli

Purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.

Shipping, Reconstitution & Storage

Appearance

Lyophilized

Shipping Conditions

Shipped at ambient temperature.

Formulation

Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.

Stability & Storage

Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

General View of Documents

Immobilized Human IL-18BP, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 26.0ng/ml determined by ELISA.

Biotinylated Human IL-18 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).

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