- Overview
- Specifications
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Overview
General Description
Recombinant Biotinylated Human IL-18 Protein (Primary Amine Labeling) is expressed from E.coli without tag.It contains Tyr37-Asp193.
Biotinylated
Yes
Specifications
Target
IL-18
Synonyms
IL-18 ; IL18 ; IGIF ; IL-1F4 ; IL-1 gamma ; IL1F4 ; IL-1g ; Interleukin 1
Accession
Species
Human
Exact Sequence
Tyr37-Asp193
Molecular Weight
The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result.
Activity
Immobilized Human IL-18BP, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 26.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
No Tag
Express System
E.coli
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Immobilized Human IL-18BP, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-18, No Tag with the EC50 of 26.0ng/ml determined by ELISA.
Biotinylated Human IL-18 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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