Identification
CAS Number
9000-86-6
Name
Alanine Aminotransferase
Synonyms
GLUTAMIC-PYRUVIC TRANSAMINASE, PORCINE H EART, ~100 U/MG ;
95675, ALANINE AMINOTRANSFERASE (CATALYT ;
glutamate-pyruvic transaminase ;
Transaminase, broad-range bacterial ;
L-Alanine :
2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT ;
Alanine-alpha-ketoglutarate aminotransferase ;
ALANINE AMINOTRANSFERASE ;
Alanine-oxaloacetate aminotransferase
Molecular Formula
C20H12Cl2N2O6
Molecular Weight
447.225
EINECS
232-561-4
Beilstein Registry Number
3562140
MDL Number
MFCD00131192
Safety Data
Personal Protective Equipment
Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our
Alanine Aminotransferase CAS 9000-86-6
General Assay Characteristics
Method | Enzymatic/RATE A/E |
Reagents | Ready to use |
Calibrators | Level 1-0.0 U/L ; Level 2-144 U/L |
Calibration Stability | 28 days |
Sample Type | EDTA,SST,Serum,Heparinised plasma |
Assay Range | 0-500U/L undiluted |
Standardisation |
Assay Principle
Proposed ALT Recipe
Reagent I
Composition | Concentration | Catalog Number |
Tris-HCl buffer | 50mM | |
L-Ala | 0.8M | |
LDH | >36KU/L | EM005 |
Tritoon X-100 | 0.01% | |
Detergent, Preservative |
Reagent II
Composition | Concentration | Catalog Number |
Tris-HCl buffer | 50mM | |
α-ketoglutarate | 95mM | |
NADH | 1.8 mM | CS001 |
Detergent, Preservative |
Major reagent ingredients made by Assay Specilist Lab are list on right
Standard&Control
Home Made : White amorphous powder, lyophilized, from Porcine liver Commercial available : RANDOX : 132U/L, Cat# HE1532
Reaction curve chart
Known Application
Commonly used in biochemical research, food preservation, clinically used as a diagnostic enzyme, determination of phosphoric acid and creatine phosphate.