
Identification
CAS Number
9000-86-6
Name
Alanine Aminotransferase
Synonyms
GLUTAMIC-PYRUVIC TRANSAMINASE, PORCINE H EART, ~100 U/MG ;
95675, ALANINE AMINOTRANSFERASE (CATALYT ;
glutamate-pyruvic transaminase ;
Transaminase, broad-range bacterial ;
L-Alanine :
2-Oxoglutarate Aminotransferase, Alanine Aminotransferase (catalytic concentration), GPT ;
Alanine-alpha-ketoglutarate aminotransferase ;
ALANINE AMINOTRANSFERASE ;
Alanine-oxaloacetate aminotransferase
Molecular Formula
C20H12Cl2N2O6
Molecular Weight
447.225
EINECS
232-561-4
Beilstein Registry Number
3562140
MDL Number
MFCD00131192
Safety Data
Personal Protective Equipment
Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter
RIDADR
NONH for all modes of transport
WGK Germany
3
Specifications and Other Information of Our
Alanine Aminotransferase CAS 9000-86-6
General Assay Characteristics
| Method | Enzymatic/RATE A/E |
| Reagents | Ready to use |
| Calibrators | Level 1-0.0 U/L ; Level 2-144 U/L |
| Calibration Stability | 28 days |
| Sample Type | EDTA,SST,Serum,Heparinised plasma |
| Assay Range | 0-500U/L undiluted |
| Standardisation |
Assay Principle

Proposed ALT Recipe
Reagent I
| Composition | Concentration | Catalog Number |
| Tris-HCl buffer | 50mM | |
| L-Ala | 0.8M | |
| LDH | >36KU/L | EM005 |
| Tritoon X-100 | 0.01% | |
| Detergent, Preservative |
Reagent II
| Composition | Concentration | Catalog Number |
| Tris-HCl buffer | 50mM | |
| α-ketoglutarate | 95mM | |
| NADH | 1.8 mM | CS001 |
| Detergent, Preservative |
Major reagent ingredients made by Assay Specilist Lab are list on right
Standard&Control
Home Made : White amorphous powder, lyophilized, from Porcine liver Commercial available : RANDOX : 132U/L, Cat# HE1532
Reaction curve chart


Known Application
Commonly used in biochemical research, food preservation, clinically used as a diagnostic enzyme, determination of phosphoric acid and creatine phosphate.









