Overview

Gen­er­al Description

Recom­bi­nant Biotiny­lat­ed Human Fc gam­ma RIIA/​CD32a (H167) Pro­tein is expressed from HEK293 with His tag and Avi tag at the C​-ter​mi​nal​.It con­tains Ala36-Ile218(H167).

Biotiny­lat­ed

Yes

Spec­i­fi­ca­tions

Tar­get

Fc gam­ma RIIA/​CD32a (H167)

Syn­onyms

CD32MGC23887 ; CDw32fc-gam­­ma-RIIa ; Fc gam­ma RIIA ; FCG2 ; Fc-gam­­ma-RIIa ; FcGR ; FCGR2 ; FCGR2A ; FCGR2A1 ; FcgRI­IA ; FCRI­IA ; fcRII-a ; IGFR2MGC30032 ; IgG Fc recep­tor II-a ; FCG2 ; CD32A

Acces­sion

P12318

Species

Human

Exact Sequence

Ala36-Ile218(H167)

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 23.2 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 30-40 kDa based on Tris-Bis PAGE result.

Activ­i­ty

The affin­i­ty con­stant of 1.44 uM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

C-His-Avi

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 5% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Biotiny­lat­ed Human Fc gam­ma RIIA (H167) , His-Avi Tag cap­tured on CM5 Chip via Anti-his anti­body can bind Rit­ux­imab with an affin­i­ty con­stant of 1.44 uM as deter­mined in SPR assay (Bia­core T200) (QC Test).

Rit­ux­imab cap­tured on CM5 Chip via Pro­tein A can bind Biotiny­lat­ed Human Fc gam­ma RIIA (H167) , His-Avi Tag with an affin­i­ty con­stant of 0.680 uM as deter­mined in SPR assay (Bia­core T200).

The puri­ty of Human Fc gam­ma RIIA (H167) is greater than 95% as deter­mined by SEC-HPLC.

Human Fc gam­ma RIIA (H167) on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

Quick Inquiry

Fill out our inquiry form and one of our experts will be in touch with you shortly.

























    Please prove you are human by select­ing the truck.