- Overview
- Specifications
- Shipping, Reconstitution & Storage
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Overview
General Description
Recombinant Biotinylated Human GITR/TNFRSF18 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gln26-Glu161.
Biotinylated
Yes
Specifications
Target
GITR/TNFRSF18
Synonyms
CD357 ; GITR ; GITR-D ; TNFRSF18 ; AITR
Accession
Species
Human
Exact Sequence
Gln26-Glu161
Molecular Weight
The protein has a predicted MW of 17.2 kDa. Due to glycosylation, the protein migrates to 26-28 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR, His Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR, His Tag with the EC50 of 0.14ug/ml determined by ELISA.
The purity of Biotinylated Human GITR is greater than 95% as determined by SEC-HPLC.
Biotinylated Human GITR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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