Overview

Gen­er­al Description

Recom­bi­nant Biotiny­lat­ed Human HLA-G Tetramer Pro­tein is expressed from HEK293 with His tag and Avi tag at the C-terminal,tetramer is assem­bled by biotiny­lat­ed monomer and strep​ta​vidin​.It con­tains Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide.

Biotiny­lat­ed

Yes

Spec­i­fi­ca­tions

Tar­get

HLA-G Tetramer

Syn­onyms

HLA G anti­gen ; sHLA-G ; b2 microglob­u­lin ; HLA G ; HLAG ; HLA-G ; MHC Class I Anti­gen G ; MHC class Ib anti­gen ; MHC-G ; sHLA-G

Acces­sion

P17693

Species

Human

Exact Sequence

Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 258 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 260-265 kDa under Non reduc­ing (N) con­di­tion based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Anti-HLA-G Anti­body, hFc Tag at 1ug/​ml (100ul/​well) on the plate. Dose response curve for Biotiny­lat­ed Human HLA-G Tetramer, His Tag with the EC50 of 19.9ng/ml deter­mined by ELISA (QC Test). The affin­i­ty con­stant of 4.38 nM as deter­mined in SPR assay (Bia­core T200). See test­ing image for detail.

Prod­uct Tag

C-His-Avi

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 5% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Human LILRB2, hFc Tag cap­tured on CM5 Chip via Pro­tein A can bind Biotiny­lat­ed Human HLA-G Tetramer, His-Avi Tag with an affin­i­ty con­stant of 4.38 nM as deter­mined in SPR assay (Bia­core T200).

Immo­bi­lized Anti-HLA-G Anti­body, hFc Tag at 1ug/​ml (100ul/​well) on the plate. Dose response curve for Biotiny­lat­ed Human HLA-G Tetramer, His Tag with the EC50 of 19.9ng/ml deter­mined by ELISA (QC Test).

Ser­i­al dilu­tions of Anti-LIL­R­B2 Anti­body were added into Biotiny­lat­ed HLA-G Com­plex Tetramer, His Tag : Human LILRB2, mFc Tag bind­ing reac­tioins. The half max­i­mal inhi­bi­tio­ry con­cen­tra­tion (IC50) is 0.29ug/ml.

Biotiny­lat­ed Human HLA-G Tetramer on Tris-Bis PAGE under Non reduc­ing (N) con­di­tion. The puri­ty is greater than 95%.

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

Quick Inquiry

Fill out our inquiry form and one of our experts will be in touch with you shortly.

























    Please prove you are human by select­ing the car.