Biotiny­lat­ed Human Siglec-3/CD33 Pro­tein, Acces­sion : P20138

• Overview
• Spec­i­fi­ca­tions
• Ship­ping, Recon­sti­tu­tion & Storage
• Gen­er­al View of Documents
• Dec­la­ra­tion
• Quick Inquiry

Overview

Gen­er­al Description

Recom­bi­nant Biotiny­lat­ed Human Siglec-3/CD33 Pro­tein is expressed from HEK293 with His tag and Avi tag at the C​-ter​mi​nal​.It con­tains Asp18-His259.

Biotiny­lat­ed

Yes

Spec­i­fi­ca­tions

Tar­get

Siglec-3/CD33

Syn­onyms

Acces­sion

P20138

Species

Human

Exact Sequence

Asp18-His259

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 29.6 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 48-58 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Anti-Siglec-3 Anti­body, hFc Tag at 0.5ug/ml (100ul/​well) on the plate. Dose response curve for Biotiny­lat­ed Human Siglec-3, His Tag with the EC50 of 40.9ng/ml deter­mined by ELISA. See test­ing image for detail.

Prod­uct Tag

C-His-Avi

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 90% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Liq­uid

Ship­ping Conditions

Shipped with blue ice.

For­mu­la­tion

Sup­plied as 0.22um fil­tered solu­tion in 20mM PB, 500mM NaCl (pH 8.0).

Recon­sti­tu­tion

Sta­bil­i­ty & Storage

Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the pro­tein into small­er quan­ti­ties for opti­mal stor­age. Please avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

Immo­bi­lized Anti-Siglec-3 Anti­body, hFc Tag at 0.5ug/ml (100ul/​well) on the plate. Dose response curve for Biotiny­lat­ed Human Siglec-3, His Tag with the EC50 of 40.9ng/ml deter­mined by ELISA.

The puri­ty of Biotiny­lat­ed Human Siglec-3 is greater than 90% as deter­mined by SEC-HPLC.

Biotiny­lat­ed Human Siglec-3 on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

Dec­la­ra­tion

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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