20210913100740 600x400 - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913

Overview

Gen­er­al Description

Recom­bi­nant Biotiny­lat­ed Human WT-1 (HLA-A*02:01) Pro­tein is expressed from HEK293 with His tag and Avi tag at the C​-ter​mi​nal​.It con­tains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMF­P­NAPYL peptide.

Biotiny­lat­ed

Yes

Spec­i­fi­ca­tions

Tar­get

WT-1 (HLA-A*02:01)

Syn­onyms

MHC ; WT1 ; RMF ; AWT1 ; GUD ; NPHS4GUD ; WAGR ; WIT-2 ; WT33

Acces­sion

A0A140T913

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMF­P­NAPYL peptide

Mol­e­c­u­lar Weight

The pro­tein has a pre­dict­ed MW of 50.5 kDa. Due to gly­co­sy­la­tion, the pro­tein migrates to 55-60 kDa based on Tris-Bis PAGE result.

Activ­i­ty

Immo­bi­lized Biotiny­lat­ed Human WT-1 (HLA-A*02:01) at 2ug/​ml (100ul/​Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Anti­body, hFc Tag with the EC50 of 63.3ng/ml deter­mined by ELISA. See test­ing image for detail.

Prod­uct Tag

C-His-Avi

Express Sys­tem

HEK293

Puri­ty

> 95% as deter­mined by Tris-Bis PAGE;> 95% as deter­mined by HPLC

Endo­tox­in

Less than 1EU per ug by the LAL method.

Ship­ping, Recon­sti­tu­tion & Storage

Appear­ance

Lyophilized

Ship­ping Conditions

Shipped at ambi­ent temperature.

For­mu­la­tion

Lyophilized from 0.22um fil­tered solu­tion in PBS (pH 7.4). Nor­mal­ly 8% tre­halose is added as pro­tec­tant before lyophilization.

Recon­sti­tu­tion

Cen­trifuge tubes before open­ing. Recon­sti­tut­ing to a con­cen­tra­tion more than 100 ug/​ml is rec­om­mend­ed. Dis­solve the lyophilized pro­tein in dis­tilled water.

Sta­bil­i­ty & Storage

Recon­sti­tut­ed pro­tein sta­ble at -80°C for 12 months, 4°C for 1 week. Use a man­u­al defrost freez­er and avoid repeat­ed freeze-thaw cycles.

Gen­er­al View of Documents

20210913100740 - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913

Immo­bi­lized Biotiny­lat­ed Human WT-1 (HLA-A*02:01) at 2ug/​ml (100ul/​Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Anti­body, hFc Tag with the EC50 of 63.3ng/ml deter­mined by ELISA.

HPLC BIO(2) - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913

The puri­ty of Biotiny­lat­ed Human WT-1 (HLA-A*02:01) was greater than 95% as deter­mined by SEC-HPLC.

20220322162434 - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913

Biotiny­lat­ed Human WT-1 (HLA-A*02:01) on Tris-Bis PAGE under reduced con­di­tion. The puri­ty is greater than 95%.

validation images - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913

validation images - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913

validation images - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913

This prod­uct is brand­ed by ChemWhat and sold through our web­site. (ChemWhat Offi­cial Web­site).

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