![Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913 20210913100740 600x400 - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913](https://www.watson-int.com/wp-content/uploads/2023/07/20210913100740-600x400.jpg)
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Overview
General Description
Recombinant Biotinylated Human WT-1 (HLA-A*02:01) Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and RMFPNAPYL peptide.
Biotinylated
Yes
Specifications
Target
WT-1 (HLA-A*02:01)
Synonyms
MHC ; WT1 ; RMF ; AWT1 ; GUD ; NPHS4GUD ; WAGR ; WIT-2 ; WT33
Accession
Species
Human
Exact Sequence
Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and RMFPNAPYL peptide
Molecular Weight
The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
Activity
Immobilized Biotinylated Human WT-1 (HLA-A*02:01) at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 63.3ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Shipping, Reconstitution & Storage
Appearance
Lyophilized
Shipping Conditions
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
General View of Documents
![Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913 20210913100740 - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913](/wp-content/uploads/validation-images/20210913100740.jpg)
Immobilized Biotinylated Human WT-1 (HLA-A*02:01) at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 63.3ng/ml determined by ELISA.
![Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913 HPLC BIO(2) - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913](/wp-content/uploads/validation-images/HPLC-BIO(2).jpg)
The purity of Biotinylated Human WT-1 (HLA-A*02:01) was greater than 95% as determined by SEC-HPLC.
![Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913 20220322162434 - Biotinylated Human WT-1 (HLA-A*02:01) Protein, Accession: A0A140T913](/wp-content/uploads/validation-images/20220322162434.jpg)
Biotinylated Human WT-1 (HLA-A*02:01) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Declaration
This product is branded by ChemWhat and sold through our website. (ChemWhat Official Website).
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